New Larix-associated Suillus species (Boletales, Agaricomycetes) from North Asia

Data Records

The data in this occurrence resource has been published as a Darwin Core Archive (DwC-A), which is a standardized format for sharing biodiversity data as a set of one or more data tables. The core data table contains 13 records.

This IPT archives the data and thus serves as the data repository. The data and resource metadata are available for download in the downloads section. The versions table lists other versions of the resource that have been made publicly available and allows tracking changes made to the resource over time.

Versions

The table below shows only published versions of the resource that are publicly accessible.

How to cite

Researchers should cite this work as follows:

Zvyagina E, Sazanova N (2024). New Larix-associated Suillus species (Boletales, Agaricomycetes) from North Asia. Version 1.5. Yugra State University Biological Collection (YSU BC). Occurrence dataset. http://ipt.ugrasu.ru:8080/resource?r=kovalenkoi&v=1.5

Rights

Researchers should respect the following rights statement:

The publisher and rights holder of this work is Yugra State University Biological Collection (YSU BC). This work is licensed under a Creative Commons Attribution Non Commercial (CC-BY-NC 4.0) License.

GBIF Registration

This resource has been registered with GBIF, and assigned the following GBIF UUID: 5e2d1104-a25f-40ab-8a99-d8426a27b65e.  Yugra State University Biological Collection (YSU BC) publishes this resource, and is itself registered in GBIF as a data publisher endorsed by Participant Node Managers Committee.

Keywords

Occurrence; Specimen

Contacts

Elena Zvyagina

• Metadata Provider ●
• Originator ●
• Point Of Contact

researcher

Yugra State University

Chekhova street, 16

628012 Khanty-Mansiysk

Khanty-Mansi Autonomous Okrug-Yugra

RU

mycena@yandex.ru

http://orcid.org/0000-0003-2063-4847

Nina Sazanova

• Metadata Provider ●
• Originator

Herbarium curator of fungi

Institute of Biological Problems of the North of the Far East Branch of the Russian Academy of Sciences

Portovaja Street, 18

685000 Magadan

Magadan Region

nsazanova_mag@mail.ru

http://orcid.org/0000-0002-0788-1942

Nina Filippova

• Curator

senior researcher

Yugra State University

Stroiteley street, 2

628508 Khanty-Mansiysk

Khanty-Mansi Autonomous Okrug-Yugra

RU

filippova.courlee.nina@gmail.com

https://fungariumysu.org/

http://orcid.org/0000-0002-9506-0991

Geographic Coverage

Specimens originated from Italia (Suillus bresadolae f. flavogriseus (Cazzoli & Consiglio) Klofac MCVE18244 Ex Herb Simonini N1844) and Russian Federation from Altay Mountains (Suillus nueschii var. caerulescens Singer LE4547, LE4554), Irkutsk Region (Suillus sp. LE18056, LE18089, LE18421, LE4766) and Magadan Region (Suillus sp. MAG5111, MAG2642, MAG1307, MAG1308, Suillus gastroflavus Zvyagina, Rebriev, Sazanova & E.F. Malysheva Holotype MAG3480 and Paratype MAG5122/LE312702)

Taxonomic Coverage

13 specimens, 5 species, 1 variety, 1 form

Temporal Coverage

Project Data

The new laboratory of molecular methods for biodiversity research and monitoring was organized thanks to the support of the Russian Ministry of Sience and Education grant for organization of New Young Researcher Laboratories as part of the implementation of the national project Science and Universities.

The personnel involved in the project:

Nina Filippova

• Curator

http://orcid.org/0000-0002-9506-0991

Sampling Methods

Collections were made, documented and preserved using the standard methods (Bondartsev, Zinger, 1955, Tulloss, 1999, Halling, 1996)

Method step description:

1. Specimens were photographed in situ, examined for taste and smell, dried at temperatures below 50°C.
2. Macroscopic descriptions were based on the study of both fresh and dried materials as well as on photographs.
3. Microstructures were observed at ×400 and ×1000 in squash preparations in 5% KOH, Congo Red, and Melzer’s reagent. Up to 30 basidiospores, 10 hymenial cystidia, and 10 terminal elements of the pileipellis per specimen were measured to obtain descriptive statistics.
4. Measurements were made in ADFImageCapture calibrated by OMP object-micrometre (LOMO). Dimensions are given as (absolute min) average min–average max (absolute max) of the all examined specimens, Q = average min–average max quotient (length/width ratio).
5. PCR ITS products were obtained without DNA extraction using the standard protocol of Thermo Scientific Phire Tissue Direct PCR Master Mix kit.
6. For amplification of the ITS region the primers ITS1-F, ITS4-B (Gardes, Bruns, 1993) were used.
7. LSU (28S) was amplified with LR0R, LR5 (Vilgalys, Hester, 1990) pair of primers, TEF1 with EF1-983F and EF1-1567R (Rehner, Buckley, 2005).
8. Amplified products were sequenced using BigDyeH Terminator 3.1 Cycle Sequencing Kit (Applied Biosystems, Foster City, California).
9. The sequences were assembled in CodonCode Aligner V.9.0.1 (CodonCode Corporation).

Collection Data

Bibliographic Citations

1. Gardes, M., Bruns, T.D. (1993) ITS primers with enhanced specifity for Basidiomycetes: application to identification of mycorrhizae and rusts. Mol. Ecol. 2: 113–118.
2. Vilgalys, R., Hester, M. (1990) Rapid genetic identification and mapping of enzymatically amplified ribosomal DNA from several Cryptococcus species. Journal of Bacteriology 172: 4239–4246.
3. Rehner, S.A., Buckley, E. (2005) A Beauveria phylogeny inferred from nuclear ITS and EF1-α sequences: Evidence for cryptic diversification and links to Cordyceps teleomorphs. Mycologia 97: 84–98. http://dx.doi.org/10.3852/mycologia.97.1.84
4. Halling, R. E. (1996) Recommendations for collecting mushrooms for scientific study. Pp. 135-141. In: Alexiades, M. N. and J. W. Sheldon (eds.), Selected Guidelines for Ethnobotanical Research: A Field Manual. The New York Botanical Garden Press, Bronx.
5. Tulloss, R. (1999) Trial Year Notebook for the Great Smoky Mountains National Park All Taxa Biodiversity Inventory. Epigeous Macromycetes. 75 p.
6. Bondartsev, A.S., Singer R.A. (1950) Guidelines for the collection of higher basidial fungi for their scientific study // Proceedings of the Komarov Botanical Institute Academy of Sciences of the USSR. V 2. ( 6). P. 499–572.

Additional Metadata